你的位置:首页 > 技术支持 > 技术交流

小动物手术保温的重要性

2017-5-27 17:25:46      点击:
动物手术保温与动物伦理和福利

动物术中和术后保温是动物实验过程中的一个至关重要的环节,是否做好保温措施直接关系到实验的成败,同时也是科研工作者在进行生命科学相关研究中将实验动物伦理和福利付诸实践的真正体现,是对动物爱心的社会责任感的体现。善待活着的动物,减少动物死亡的痛苦!!!



动物手术保温的科学价值

Responsible scientists know that good science and good animal care go hand in hand, and would not tolerate cruel or inhumane treatment of any laboratory animal.

------The U.S. Animal Welfare Act

美国动物福利法案申明指出“负责的科学家深知,好科学与善待动物是相辅相成的,我们不能容忍残杀或不人道的对待任何实验动物。”


保障动物实验结果准确性:科研效果更好、结果可信、更加客观真实


动物手术低体温的危害


为什么需要对手术动物进行保温?

  1. 1.损害机体免疫功能,减少皮肤血流和氧供,增加伤口感染率

  2. 2.降低血流循环和血液指标,导致凝血发生,非常不利于血液或血流相关指标监测和测定,比如测量血流量、血压等

  3. 3.降低药物在机体组织中的代谢速度,影响机体代谢

  4. 4.延长麻醉恢复时间,可能明显增加动物死亡率

  5. 5.引起其他疾病的发生,比如心血管并发症

  6. 6.无法得到真实或接近真实的实验数据

  7. More………


动物手术低体温的常见原因
  • 1.麻醉剂的作用:麻醉剂导致扩张血管、抑制体温调节,从而导致体温下降

  • 2.手术室的低温环境,尤其是普通动物室实验环境

  • 3.手术台的“冷”材质,比如不锈钢、树脂材质的台面

  • 4.长时间药物注射:长时间输入与环境等温的液体,对动物体液造成“冷稀释”作用,从而导致动物体温下降

  • 5.皮肤消毒剂的使用,尤其是裸露皮肤大面积的消毒,导致体内热量散失

  • 长时间的手术

  • 6.创伤较大的开胸、开腹手术,造成热量散发较多

  • 7.动物自身年龄、疾病因素,如营养不良、肿瘤模型手术

  • Others………

动物手术保温的常见措施




Solution 1

Solution 2


动物手术保温的规范操作


术中


1.如果使用电热毯保温方式,切忌电热毯直接放置于不锈钢、树脂等“冷”材质手术台面,因这些材质传热会导致电热毯加热很慢甚至无法升温到预期温度;
2.除手术部位外,其他部位尽量不暴露,可以使用布巾遮盖;
3.如果是注射液体或药物,且实验时间较长,必要时,可以同时对输进动物体内的液体进行预热;
4.手术操作配合熟练,减少手术时间;
5.注意体温监测。



术后


  • 继续将动物放于保温毯或保温台上,待动物恢复。



动物手术麻醉、保温全套方案


Solution 1

此套方案集吸入麻醉、立体定位和动物手术保温全套功能于一体,是从事小鼠、大鼠等小动物神经科学相关研究的标准配置,主要应用范围:

  1. 1.光遗传

  2. 2.神经电生理

  3. 3.钙成像

  4. 4.微透析

  5. 5.颅脑&脊髓损伤

  6. 6.颅内给药

  7. and more……..

Solution 2

此套方案集吸入麻醉、动物身体固定和手术保温全套功能于一体,是从事小鼠、大鼠等小动物常用实验的标准配置,主要应用范围:

  1. 1.动物解剖、分离

  2. 2.脑梗、心梗等模型造模

  3. 3.心脏、尾静脉、静脉等缺血

  4. 4.颈内静脉和尾静脉给药

  5. 5.动物活体成像

  6. and more……..




瑞沃德品牌体温维持仪引用文献(部分):


  1. 1.Li L, Tang Y, Sun L, et al. In vivo fiber photometry of neural activity in response to optogenetically manipulated inputs in freely moving mice[J]. Journal of Innovative Optical Health Sciences, 2017: 1743001.

  2. 2.Zhu S Z, Gu Y, Wu Z, et al. Hypothermia followed by rapid rewarming exacerbates ischemia-induced brain injury and augments inflammatory response in rats[J]. Biochemical and biophysical research communications, 2016, 474(1): 175-181.

  3. 3.Chernyuk D P, Vlasova O L, Bezprozvanny I B, et al. Hyperexpression of STIM2 protein lowers the amount of Abeta plaques in the brain of Alzheimer's disease mouse model[J]. St. Petersburg Polytechnical University Journal: Physics and Mathematics, 2016, 2(4): 329-336.

  4. 4.Xin Y, Li Z, Wu W, et al. Recognition unit-free and self-cleaning photoelectrochemical sensing platform on TiO 2 nanotube photonic crystals for sensitive and selective detection of dopamine release from mouse brain[J]. Biosensors and Bioelectronics, 2017, 87: 396-403.

  5. 5.Gu H, Yang Y, Zhou X, et al. Online electrochemical method for continuous and simultaneous monitoring of glucose and l-lactate in vivo with graphene hybrids as the electrocatalyst[J]. Journal of Electroanalytical Chemistry, 2014, 730: 41-47.

  6. 6.Huang L, Yuan T, Tan M, et al. A retinoraphe projection regulates serotonergic activity and looming-evoked defensive behaviour[J]. Nature Communications, 2017, 8.

  7. 7.Yue X H, Tong J Q, Wang Z J, et al. Steroid sulfatase inhibitor DU-14 protects spatial memory and synaptic plasticity from disruption by amyloid β protein in male rats[J]. Hormones and behavior, 2016, 83: 83-92.

  8. 8.Tang R. In vivo imaging of lung tumor growth using in-line x-ray phase contrast technique.(2016) J Imag-ing Sci 1 (1): 1-3[J]. J Imaging Sci, 2016, 1(1).

  9. 9.Zhang Q, Lan Y, He X, et al. Allopurinol protects against ischemic insults in a mouse model of cortical microinfarction[J]. Brain research, 2015, 1622: 361-367.

  10. 10.Lin X, Miao P, Wang J, et al. Surgery-related thrombosis critically affects the brain infarct volume in mice following transient middle cerebral artery occlusion[J]. PloS one, 2013, 8(9): e75561.

  11. 11.Cai J, He C, Chen L, et al. Melatonin mitigate cerebral vasospasm after experimental subarachnoid hemorrhage: a study of synchrotron radiation angiography[J]. Journal of Instrumentation, 2013, 8(06): C06004.

  12. 12.Liu T, Zhang T, Yu H, et al. Adjudin protects against cerebral ischemia reperfusion injury by inhibition of neuroinflammation and blood-brain barrier disruption[J]. Journal of neuroinflammation, 2014, 11(1): 107.

  13. 13.Pan R, Cai J, Zhan L, et al. Buyang Huanwu decoction facilitates neurorehabilitation through an improvement of synaptic plasticity in cerebral ischemic rats[J]. BMC complementary and alternative medicine, 2017, 17(1): 173.

  14. 14.Tang M, Lin W, Pan Y, et al. Hippocampal neurogenesis dysfunction linked to depressive-like behaviors in a neuroinflammation induced model of depression[J]. Physiology & behavior, 2016, 161: 166-173.

  15. 15.Yan A, Zhang T, Yang X, et al. Thromboxane A2 receptor antagonist SQ29548 reduces ischemic stroke-induced microglia/macrophages activation and enrichment, and ameliorates brain injury[J]. Scientific reports, 2016, 6.

  16. 16.Wang R Y, Lin X J, Yang G Y, et al. Effect of hirulog-like peptide on middle cerebral artery occlusion-induced brain injury in mice[J]. Neuroscience, 2014, 277: 568-576.

  17. 17.Wang Y, Zhang Y, Huang J, et al. Increase of circulating miR-223 and insulin-like growth factor-1 is associated with the pathogenesis of acute ischemic stroke in patients[J]. BMC neurology, 2014, 14(1): 77.

  18. 18.Li Q, Zhang T, Wang J, et al. Rapamycin attenuates mitochondrial dysfunction via activation of mitophagy in experimental ischemic stroke[J]. Biochemical and biophysical research communications, 2014, 444(2): 182-188.

  19. 19.He X, Lu Y, Lin X, et al. Optical inhibition of striatal neurons promotes focal neurogenesis and neurobehavioral recovery in mice after middle cerebral artery occlusion[J]. Journal of Cerebral Blood Flow & Metabolism, 2016: 0271678X16642242.

  20. 20.Miao P, Wu Z, Li M, et al. Synchrotron Radiation X-Ray Phase-Contrast Tomography Visualizes Microvasculature Changes in Mice Brains after Ischemic Injury[J]. Neural plasticity, 2016, 2016.

  21. 21.Tang G, Liu Y, Zhang Z, et al. Mesenchymal stem cells maintain blood‐brain barrier integrity by inhibiting aquaporin‐4 upregulation after cerebral ischemia[J]. Stem Cells, 2014, 32(12): 3150-3162.

  22. 22.Mu Z H, Jiang Z, Lin X J, et al. Vessel dilation attenuates endothelial dysfunction following middle cerebral artery occlusion in hyperglycemic rats[J]. CNS neuroscience & therapeutics, 2016.

  23. 23.Wang Z, Song F, Li J, et al. PET demonstrates functional recovery after treatment by Danhong injection in a rat model of cerebral ischemic-reperfusion injury[J]. Evidence-Based Complementary and Alternative Medicine, 2014, 2014.

  24. 24.Lin X, Miao P, Mu Z, et al. Development of functional in vivo imaging of cerebral lenticulostriate artery using novel synchrotron radiation angiography[J]. Physics in medicine and biology, 2015, 60(4): 1655.

  25. 25.Miao P, Lin X, Feng S, et al. In-vivo brain blood flow imaging based on laser speckle contrast imaging and synchrotron radiation microangiography[J]. Laser Physics, 2014, 24(8): 085603.

  26. 26.Lv B, Li F, Han J, et al. Hif-1α overexpression improves transplanted bone mesenchymal stem cells survival in rat MCAO stroke model[J]. Frontiers in Molecular Neuroscience, 2017, 10.

  27. 27.Liu Y, Tang G H, Sun Y H, et al. The protective role of Tongxinluo on blood–brain barrier after ischemia–reperfusion brain injury[J]. Journal of Ethnopharmacology, 2013, 148(2): 632-639.

  28. 28.Tang Y, Cai B, Yuan F, et al. Melatonin pretreatment improves the survival and function of transplanted mesenchymal stem cells after focal cerebral ischemia[J]. Cell transplantation, 2014, 23(10): 1279-1291.

  29. 29.Li Q, Tang G, Xue S, et al. Silica-coated superparamagnetic iron oxide nanoparticles targeting of EPCs in ischemic brain injury[J]. Biomaterials, 2013, 34(21): 4982-4992.

  30. 30Tang Y, Cai B, Yuan F, et al. CT-0998 Accepted 04/19/2013 for publication in “Cell Transplantation” Melatonin pretreatment improves the survival and function of transplanted mesenchymal stem cells after focal cerebral ischemia[J]. 2013.

  31. 31.Yue X H, Tong J Q, Wang Z J, et al. Steroid sulfatase inhibitor DU-14 protects spatial memory and synaptic plasticity from disruption by amyloid β protein in male rats[J]. Hormones and behavior, 2016, 83: 83-92.

  32. 32.Yao C, Zhang J, Liu G, et al. Neuroprotection by (-)-epigallocatechin-3-gallate in a rat model of stroke is mediated through inhibition of endoplasmic reticulum stress[J]. Molecular medicine reports, 2014, 9(1): 69-72.

  33. 33.Falei Yuan, Yaohui Tang, Xiaojie Lin, Yan Xi, Yongjing Guan, Tiqiao Xiao, Jun Chen, Zhijun Zhang, Guo-Yuan Yang, and Yongting Wang.Optimizing Suture Middle Cerebral Artery Occlusion Model in C57BL/6 Mice Circumvents Posterior Communicating Artery Dysplasia. Journal of Neurotrauma. May 1, 2012, 29(7): 1499-1505. 

  34. 34.Nathalie Cloutier, Alexandre Paré, Richard W. Farndale, H. Ralph Schumacher, Peter A. Nigrovic, Steve Lacroix, and Eric Boilard. Platelets can enhance vascular permeability. Blood August 9, 2012, vol. 120 no. 6 1334-1343. 

  35. 35.Jun Cai, Chuan He, Falei Yuan, Lujia Chen, Feng Ling. A novel haemodynamic cerebral aneurysm model of rats with normal blood pressure. Journal of Clinical Neuroscience, January 2012, Volume 19, Issue 1, Pages 135–138. 

  36. 36.Bohua Xie, Peng Miao, Yuhao Sun, Yongting Wang, Guo-Yuan Yang. Micro-Computed Tomography for Hemorrhage Disruption of Mouse Brain Vasculature. Translational Stroke Research, July 2012, Volume 3, Issue 1 Supplement, pp 174-179. 

  37. 37.Rongbiao Tang, Yan Xi, Wei-Min Chai, Yongting Wang, Yongjing Guan, Guo-Yuan Yang, Honglan Xie and Ke-Min Chen. Microbubble-based synchrotron radiation phase contrast imaging: basic study and angiography applications. Phys. Med. Biol., 2011, Vol 56:  3503.

  38. 38.Mingfeng Xia, Minjuan Bian, Qian Yu, Jie Liu, Yufang Huang, Xueting Jin, Shiduo Lu, Mei Yu and Fang Huang. Cold water stress attenuates dopaminergic neurotoxicity induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine in mice. Acta Biochim Biophys Sin, 2011,43 (6): 448-454.

  39. 39.Cai J, Sun Y, Yuan F, Chen L, He C, Bao Y, Chen Z, Lou M, Xia W, Yang G and Ling F. A Novel Intravital Method to Evaluate Cerebral Vasospasm in Rat Models of Subarachnoid Hemorrhage: A Study with Synchrotron Radiation Angiography. PLoS ONE,2012, 7(3): e33366.

  40. 40.Bin Cai, Yi Lin, Xie-Hua Xue, Ling Fang, Ning Wang, Zhi-Ying Wu. TAT-mediated delivery of neuroglobin protects against focal cerebral ischemia in mice. Experimental Neurology, January 2011, Volume 227, Issue 1, Pages 224–23。

相关产品
暂无相关产品。
相关信息
    暂无相关信息。